Aspirating And Dispensing Liquids - HTL LABMATE pro LMP2 Manual De Instrucciones

Pipettes LMP1000, LMP5000,
Red figures at the top = ml
Increment =
Pipettes LMP10000
Red figures at the top = ml
Increment = 20 μl
The volume of the pipette is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the black adjustment
knob (Fig. 1B). To attain the maximum accuracy, set vol-
ume must be approached from a higher value by dimin-
ishing counter readings.
• If the desired volume is lower than set volume shown
by the counter, the operator should turn the pipetting
pushbutton (Fig. 1A2) or the black adjustment knob
(Fig. 1B) to the direction diminishing counter readings
to the required volume. Before achieving the required
volume slowly rotate the knob and observe carefully
diminishing reading to avoid accidentally passing the
setting value.
• If the desired volume is higher than set volume shown
by the counter, the operator should turn the pipetting
pushbutton (Fig. 1A2) or the black adjustment knob
(Fig. 1B) increasing the value until the lower figure
wheel comes 1/3 of a turn beyond the required setting
and then slowly backwards until the setting reaches the
desired volume. Make sure not to pass the setting
value.
If the knob is accidentally turned too far, the process
must be repeated. The desired volume must always be
set from the higher value in the order of decreasing value.

3 - ASPIRATING AND DISPENSING LIQUIDS

Place a tip on the shaft of the pipette. See Section 6 for
the appropriate tip. Press the tip on firmly using a slight
twisting motion to en sure a positive, airtight seal.
Important: Never aspirate liquids into the pipette
without a tip attached.
5
LMP1000 LMP5000
2 μl 10 μl
LMP10000
Aspiration
Press the pushbutton to the first positive stop, (Fig. 2A).
Holding the pipette vertically, immerse the tip into the
sample liquid. The depth to which the tip is immersed in
the sample liquid depends on the model.
Model
LMP2
LMP10
LMP20, LMP50, LMP100
LMP200, LMP250, LMP1000
LMP5000
LMP10000
Release the pushbutton slowly and smoothly to aspirate
the sample, (Fig. 2B). Wait one second and then with-
draw the tip from the liquid. When the pipette tip is
immersed not as deeply as the recommended depth or
when the pipetting pushbutton is rapidly released air
may enter the disposable tip.
Avoid touching the orifice of the tip.
Dispensing
• Place the end of the tip against the inside wall of the
vessel at an angle of 10 to 40 degrees.
• Press the pushbutton smoothly to the first stop, (Fig. 2C).
Wait one second.
• Press the pushbutton to the second stop to expel any
remaining liquid, (Fig. 2D).
• Keeping the pushbutton depressed to the very end,
remove the pipette by drawing the tip against the inside
surface of the receiving vessel.
• Release the pushbutton to its starting position, (Fig. 2E).
• Eject the tip by pressing the tip ejector button, (Fig. 2F).
Remember to change the tip whenever a different kind
of liquid is to be sampled.
Filters
Replaceable filter installed in a seat in the bottom part of
the shaft is used in 5000 μl and 10000 μl pipettes (Fig. 3L).
The filter prevents the aspirated liquid from entering into
the shaft and thus from polluting the inside of the shaft
and the piston. Using the filter is especially important
when aspirating and dispensing large volumes of liquid.
If the filter becomes wet during liquid aspiration it should
be replaced with a new one.
ENGLISH
Immersion depth (mm)
≤ 1
≤ 1
2 ÷ 3
2 ÷ 3
3 ÷ 6
5 ÷ 7
6