Phase Contrast - Optika Italy IM-5 Serie Manual De Instrucciones

8.10 Phase contrast

Phase contrast slider
Adjustable phase slider.
● The light ring is pre-centered when the
microscope leaves the factory. It should
therefore need no further adjustment. If
a recentering is needed, it can be per-
formed via the two side bolts.
● The 4x/10x position ① must be used with
4x and 10x phase contrast objectives, the
20x/40x position ② with the 20x and 40x
and the SL position ③ is used for brigh-
field. (Fig.34)
Installing the phase contrast slider
1. Insert the slider into the condenser, printed
face up.
2. Pull the slider into the desired position, to
the click stop.
3. When in phase contrast observation, keep
the aperture diaphragm adjustment lever
on the "O" (open) position. (Fig.35)
SLIDER POSITION
SL
4x/10x
20x/40x
8.11 Darkfield
Darkfield slider (Fig. 36)
Installing the darkfield slider
1. Insert the slider into the condenser, printed
face up.
2. Move the slider into position "DF" ② to
perform darkfield observation.
3. Move the slider into empty position ③ to
perform brightfield observation.
4. When in darkfield observation, keep the
aperture diaphragm adjustment lever on
the "O" (open) position.
● Darkfield observation is possible with
objectives having N.A. up to 0.40.
With higher N.A. the darkfield effect
●
cannot be achieved because of the
condenser N.A.
①
MEANING
empty hole
phase ring 4x/10x
phase ring 20x/40x
Page 13
② ③
APPLICATION
brightfield observation
phase contrast observation with 4x and
10x objectives
phase contrast observation with 20x and
40x objectives
③
②
Fig.34
Fig.35
Fig.36