Centering The Phase Ring - Optika Italy IM-5 Serie Manual De Instrucciones

8.12

Centering the phase ring

► Usually this operation is not needed.
If necessary, please proceed with the
following steps:
1. Place a specimen on the stage and focus
it.
2. Remove the eyepiece from the sleeve,
and replace it with the centering telescope
(CT). (Fig.37)
3. Check that the phase ring and the objective
match, and that both are steadily set on a
click stop.
4. Use the CT to focus on the phase annulus's
image ① and the phase contrast ring's
image ②. If the phase annulus's image is
not sharp, adjust the CT until you can see a
clear image of the phase annulus. (Fig. 38)
5. Adjust the bolts of the two centering
holes in the phase contrast slider using
the centering screws ③ until the phase
annulus center and the phase contrast ring
center coincide. (Fig. 39)
6. The 4x and the 10x phase contrast
objectives use the same ring on the
phase contrast slider. The coincidence of
the phase annulus center and the phase
contrast center must be verified with both
objectives.
► If the phase annulus is incorrectly
centered, the contrast will be severely
impaired.
► The phase ring may need recentering
during and after observation of very
thick specimens.
► The phase ring may show an apparent
misalignment if the cover glass is not
flat.
③
②
Page 14
①
Fig.37
Fig.38
Fig.39