Brightfield Observation (Bf); Phase Contrast Observation (Ph) - Optika Italy B-510 Serie Manual De Instrucciones

11.1 Brightfield Observation (BF)
1. Move the condenser slider all the way toward left
to insert the empty position. (Fig. 56)
2. Now repeat the steps described in the chapter
"Brightfield observation procedure".

11.2 Phase Contrast Observation (PH)

1. Center the condenser as already described in the
chapter 9.8.
• This condenser does not have a swing-out
lens, so the operation described in step 2 is not
necessary.
2. Move the condenser slider all the way toward
right to insert the phase ring dedicated to 40X
objective. (Fig. 57)
3. Insert 40x objective into the light path.
4. Open aperture diaphragm.
5. Place a specimen on the stage and focus.
6. Remove one eyepiece and insert the centering
telescope. (Fig. 49)
7. Rotate the upper part of the centering telescope
until the two phase rings (one dark and one bright)
visible in the telescope are in focus. (Fig. 49-50)
8. Using centering screws on the slider ① (Fig. 58),
center the phase rings as already descibed in the
chapter 10.3.
9. At the end remove the centering telescope,
reinstall the eyepiece and begin observation.
• With 40x objective it may be useful to slightly
raise the condenser, to obtain a better
projection of the phase rings. This is not a
defect.
• With the 4X objective, the condenser could
have a dark halo at the periphery of the field
of view. This is not to be considered a defect.
10. For observation of asbestos fibres in phase
contrast, remove the provided 10X eyepieces and
insert the 12.5X eyepieces.
①
Page 36
Fig. 56
Fig. 57
Fig. 58