Use Of Light Excluding Plate; Use Of The Microscope (B-510Ld1 / Ld2) - Optika Italy B-510 Serie Manual De Instrucciones

14.2.2 Use of light excluding plate

• Microscope is provided with a light excluding
plate that can be placed on the stage and
prevents flare and reflections coming from
the condenser front lens.
The plate can be used in two different ways.
Mode n° 1: place the plate on the stage (under the
slide holder) and place the slide directly over the
plate. (Fig. 70)
Mode n° 2: lower the condenser and insert the plate
between the two layers of the stage. (Fig. 71).
• In both cases it is possible to move the sample
using the stage X-Y translation knobs.

14.3 Use of the microscope (B-510LD1 / LD2)

1. Turn on the fluorescence LED, by moving the main
switch placed in the back side of the microscope
in the "II" position.
2. Move the filter selector ② to one of the available
positions until the click stop. (Fig. 72).
• LD1 and LD2 models have a 2-position filter
holder slide. In the case of the LD1 model, the
slide only houses a B filter, while in the LD2 model
the slide houses a B and a G filter.
FILTER EXCITATION
CUBE FILTER
B 460-490 nm
G 510-550 nm
DICHROIC EMISSION
MIRROR FILTER
505 nm 515LP nm
570 nm 575LP nm
• FITC: fluorescent antibodies
• Achridine orange: DNA, RNA
• Auramine
• Rhodamine, TRITC: fluorescent antibodies
• Propidium iodide: DNA, RNA
• RFP
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APPLICATIONS
Fig. 70
Fig. 71
Fig. 72