Simultaneous Observation In Phase Contrast + Fluorescence (B-510Fl) - Optika Italy B-510 Serie Manual De Instrucciones

15. Simultaneous observation in Phase Contrast + Fluorescence (B-510FL)

• This microscope allows observation in transmitted light Phase contrast in combination with reflected
light Fluorescence. Samples with rapid decay must first be observed in Fluorescence and then in
Phase Contrast. The combined observation allows you to easily identify some areas of the sample
that emit fluorescence.
1. Turn on the power supply for the HBO fluorescent bulb and wait 5 minutes before the arc stabilizes.
2. Move the filter selector to an empty position or, if the filter holder is complete, to the position containing the
UV filter.
3. Insert the desired PH lens and rotate the phase contrast condenser turret to the position containing the
corresponding phase ring.
4. Focus the sample.
5. Adjust the light intensity of the transmitted light.
6. Move the fluorescence filter selector to the desired position.
7. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate
the intensity of the fluorescence with that of the phase contrast.
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