Possible faults of the microscope and methods of their elimination
External manifestation of
the fault
Cut or uneven illumination
There is dust, dirt in the
field
Poor quality of object
image (low resolution, poor
contrast)
Object images when
observed with two eyes in
two eyepieces do not match
When switching the low
magnification objective
lens to higher magnification
objective lens, the
objective lens hits the
object
The halogen lamp is not on
after activation
The mercury-filled lamp
does not go on or went off
The object fluorescence
brightness has reduced
greatly
Probable cause
The revolving nosepiece is not in fixation
position (the objective lens is not on the
microscope axis)
Any objective or eyepiece lens etc. is
contaminated
The condenser is not in operating position —
too low or warped
Any lens or the stage is contaminated
There is no cover slip on the object, or its
thickness does not comply with the standard
The object is put with its cover slip down
Immersion oil got onto the frontal objective
lens. There is dry immersion oil on frontal
objective lens 100х ∞/0.17
Immersion oil was not applied on the 100x
frontal objective lens
There are bubbles in the immersion oil
The aperture diaphragm of the condenser is
open or closed too much
Eyepiece tubes of the binocular head are not
set in accordance with the observer's eye base
The stage with the object is turned over
The cover slip is too thick
The lamp is out.
The fuse blew out (safety fuse).
The power supply unit is off
The mercury-filled lamp is installed wrongly
The lamp failed — the bulb has become blurred
Tighten the revolving nosepiece and put the
objective lens into the fixed position, i.e. on
the optical axis
Visually inspect lenses and clean them
Put the condenser in the operating position
Remove dirt
Use the object with a cover slip of standard
thickness of 0.17mm
Turn the object over
Clean the immersion oil from the surfaces of
frontal objective lenses
Apply oil
Clean the immersion oil from the objective
lens, the object, the stage and reapply it
Set the necessary diaphragm size
Install the binocular head in accordance with
the instructions of subsection "Microscope
focusing for binocular observation"
Install the object with the stage up
Use the cover slip of standard thickness
Replace the lamp in accordance with the
instructions of subsection "Transmitted light
illumination system"
Disconnect the microscope from the grid and
replace the fuses
Check POWER indication on the power supply
unit casing; if it is missing, disconnect from
the grid and replace safety fuses with the new
ones from the package
Disconnect the power supply unit from the
grid, disconnect the flashlight cord from the
unit.
Remove the flashlight (after it cooled down),
check the proper installation of the lamp
according to the instructions of subsection
"Impinging light illumination system"
Replace the lamp following the instructions of
subsection "Impinging light illumination system"
17
Method of elimination