Waters HPLC Diagnóstico Y Corrección De Anomalías página 121

Column Equilibration Column Equilibration
Initially purge column with 10-20 column
volumes (V
analysis
(4.6x150mm : V
Caution : If our are using a buffer make sure it does not
precipitate in the column when initially flushing the column
w. mobile phase
— Most RP column comes in 100% organic usually acetonitrile
— Initially flush with water/organic and the switch to the mobile
phase
) mobile phase to be used in
c
V = 0.5 x D
c
= 1.6ml, Equil. = 16-32ml)
c
2
x L
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