Waters HPLC Diagnóstico Y Corrección De Anomalías página 63

•Options A and A'
•600 mL
•640 mL
• • 1000 mL 1000 mL
•Options A and A': Adding organic/aqueous directly to measuring vessel
• Need to specify which solvent is added first: e.g. MeOH to H
•Options B: Separate volumetric measurement
•
•
•Mobile Phase Composition
•is Critical for Reproducibility
•60/40 (MeOH/Water)
•440 mL
•400 mL
•Difference due to
•partial volumes*
•Note: Partial Volumes on mixing may give
• more or less than initial volumes
•Options B
• • 600 mL 600 mL
• • • • abc gr abc gr abc gr abc gr
•* 960mL
(not recommended)
O or H
2
• • 400 mL 400 mL
• • xyz gr xyz gr
• • <1000 mL <1000 mL
• • (960mL) (960mL)
O to MeOH
2
©2007 Waters Corporation 63