Waters HPLC Diagnóstico Y Corrección De Anomalías página 52

Retention time drift/ Retention time drift/
incorrect retention times incorrect retention times
Pump Flow Rate Problem
— Correct flow rate ?
— Air in pump
— — Check valve not working correctly Check valve not working correctly
Wrong Column Type:
— C8 – less retention
— C18 – more retention
Temperature Problem
— warmer – less retention,
— colder – more retention
% Organic In Mobile Phase
— more – less retention,
— less – more retention)
•More or Less Retention Time
•(All Peaks Affected)
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