Waters HPLC Diagnóstico Y Corrección De Anomalías página 93

Tips from Case Studies Tips from Case Studies
Mobile Phase Preparation can be highly critical to ultimate
method performance.
Write your method procedures clearly so that no mistakes
or misinterpretation can occur.
Sequence of mixing, and method of measurement (volume, Sequence of mixing, and method of measurement (volume,
weight) of components can dramatically change the
chromatography.
(pH control and % organic are especially important for some
methods)
Differences will often be observed for a mobile phase
prepared in a single reservoir vs. "same" mobile phase
made by blending lines using a proportioning/mixing valve
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