Waters HPLC Diagnóstico Y Corrección De Anomalías página 46

Encountered when mass injected onto column exceeds a certain limit
6.25 g injected
25 mg injected
Note that the back of the peaks of the analytical and prep loads are at the same retention (-------)
Concentration/Mass Overload Concentration/Mass Overload
– Note earlier lift-off point for preparative load
2
Minutes
2
Minutes
Analytical load of ~6 g
6
4
Preparative load of 25 mg
generates mass overload
4 6
yields efficient peak
shape
peak shape
©2007 Waters Corporation 46