Waters HPLC Diagnóstico Y Corrección De Anomalías página 36

Mixed Mixed- - mode retention mode retention
Hydrophobic Interaction with
Bonded Phase
O- Si
O- Si
Mobile
Phase pH < 3 Phase pH < 3
Si - OH
O- Si
O- Si
Base
OH
O- Si
O- Si
OH
O- Si O- Si
OH
O- Si
O- Si
O- Si
OH
Ion exchange Interaction
with Charged Sites
O-Si
O-Si
O-
O-Si
O-Si
+
)
(CH
HN
O-
(CH
3
2 2
O-Si O-Si
O-
O-Si
O-Si
O-Si
O-
O--Si
O-Si
+
) HN
3 2 3 2
Mobile
Phase pH > 3
Si – O
Base
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