This function is used to set parameters for run.
1. In the Tools screen, touch Run Set to open the screen:
4. Run Set
• Enter a 'Lid idle temperature' to let the lid heating to the desired temperature
• Enter a 'Block idle temperature' to let the block heating or cooling to this
• Enter a 'Lid temp. when running' to pre-heat lid to a desired temperature
• Enter most frequently used sample volume value in the 'Default sample
• • Enter 'If step temperature <=X°C, Lid Auto Shut Off" to automatically turn
Touch
5. SystemSet
when instrument is idle. Click the circle at left will enable this function.
temperature when instrument is idle. Click the circle at left will enable this
function.
For example, you can keep the block temperature below 20°C so that when a
program starts run, the block temperature will maintain below 20°C while the
lid is heating before run. Because the sample temperature does not increase,
so the activity of DNA polymerase is inhibited, the likelihood of nonspecific
primer binding and the yield of unwanted products such as primer-dimers are
decreased.
before running a program. We recommend you select a temperature five to ten
Celsius above the highest temperature of the program steps (e. g., the
denaturation step) to protect sample from evaporation and condensation. If
you select 'Off' on keyboard, the lid heating function will be disable when a
program is running.
volume' box.
off the lid heating function when block temperature below a X value. The
default value is 20°C.
√(Save)
to accept changes.
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