Optika Italy B-1000 Serie Manual De Instrucciones página 19

11. Use of the microscope in Fluorescence (reflected light)
11.1 LED switch on
1. Turn the main switch ①. (Fig. 32)
2. Adjust to the desired brightness by rotating the
dial ①.
11.2 Use of fluorescence
The filter turret is provided with 4 positions.
• Position "1" is free and is used for Brightfield
• Position "2" contains B (Blue) filter
• Position "3" contains G (Green) filter
• Position "4" is free and is used for Brightfield
• It is not possible to install additional fluores-
cence filters.
1. Move the filter selector ② in the desired position.
(Fig. 33)
F I L T E R EXCITATION
NAME FILTER
B 450-490 nm
G 500-540 nm
D I C H R O I C BARRIER
MIRROR FILTER
495 nm 520LP nm
560 nm 575LP nm
• FITC: fluorescent antibodies
• Achridine orange: DNA, RNA
• Auramine
• Rhodamine, TRITC: fluorescent antibodies
• Propidium Iodide: DNA, RNA
• RFP
Page 19
①
②
APPLICATIONS
F ig. 32 F ig. 32
F ig. 33 F ig. 33