Optika Italy B-1000 Serie Manual De Instrucciones página 23

14. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM
I. Optical System:
LED operates, but field of view remains
dark
Field of view is obscured or not evenly
illuminated
Dirt or dust is visible in the field of view Dirt/dust on the eyepieces
Image looks double
Low image quality
• Image is not sharp
• Contrast is poor
• Details are indistinct
• Image glares
CAUSE
Brightness is too low
Aperture and field diaphragms are not
opened wide enough
Condenser is lowered too much.
Fluorescence filter selector is not in a
click stop
Fluorescence filter is not suitable for
the specimen
Light path selector knob is set to the
camera position
Light path selector knob is in an inter-
mediate position
Revolving nosepiece is not correctly
engaged
Condenser is not attached properly
Revolving nosepiece is not attached
properly
An objective that falls outside of the
condenser's illumination range is used
Condenser is not properly centered
Field iris diaphragm is stopped down
too far
Fluorescence filter selector is not in a
click stop
Dirt/dust on the condenser surface
Dirt/dust on the specimen
Aperture iris diaphragm is stopped
down too far
The condenser is not well centered or
it is in a wrong height
Condenser is lowered too much
Aperture diaphragm is stopped down
too far
Revolving nosepiece is not mounted
properly
Front lens of objective is dirty
Immersion oil is not being used with an
oil immersion objective
Immersion oil contains bubbles
Recommended immersion oil is not
used
For transmitted light observation, cov-
erglass thickness must not exceed
0.17 mm
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SOLUTION
Set brightness to a proper level
Adjust them to proper sizes
Adjust the condenser height position
Move the selector to a click stop
Use a suitable filter
Move the knob to the eye position
Set the knob according to the observa-
tion method
Make sure that the revolving nose-
piece clicks properly into place
Re-attach it
Push the side dovetail all the way until
it is stopped
Use a condenser to match the purpose
Center the condenser
Open the field iris diaphragm until it cir-
cumscribes the field
Move the selector to a click stop
Clean thoroughly
Open aperture iris diaphragm
Set the condenser according to Koe-
hler settings
Adjust the condenser height position
Open aperture diaphragm
Push the slide dovetail all the way until
it is stopped
Clean objective
Use immersion oil
Remove the bubbles
Use the provided immersion oil
Use a coverglass with thickness 0.17
mm