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Idiomas disponibles
Idiomas disponibles
AN EXAMPLE FOR EACH
OF THESE PIPETTORS IS GIVEN BELOW
Counter
Model
readings
—
|
|
1
—
|
|
VWR2
2
—
|
|
5
—
—
|
|
0
—
|
|
VWR10
7
—
|
|
5
—
—
|
|
1
—
|
|
VWR20
2
—
|
|
5
—
—
|
|
0
—
|
|
VWR100
7
—
|
|
5
—
—
|
|
1
—
|
|
VWR200
2
—
|
|
5
—
—
|
|
0
—
|
|
VWR1000
7
—
|
|
5
—
—
|
|
1
—
|
|
VWR5000
2
—
|
|
5
—
—
|
|
0
—
|
|
VWR10000
7
—
|
|
5
—
The volume of the pipettor is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the adjustment knob
(Fig. 1B).
To attain the maximum accuracy, set volume must be
approached from a higher value. If the desired value is
lower than the previous value, simply use the knob in the
pushbutton (Fig. 1A2) or the adjustment knob (Fig. 1B)
to adjust the value down to 1/3 turn above the required
setting. If the desired value is higher than the previous
value, use the knob in the pushbutton or the adjustment
knob to adjust the value to 1/3 turn above the required
value. The setting should then be performed in the fol-
lowing manner:
With the volume set to 1/3 above the required setting,
slowly turn the pipetting pushbutton knob or the adjust-
ment knob to the required volume.
12
Set
Increment
volume
1.25 μl
0.002 μl
7.5 μl
0.02 μl
12.5 μl
0.02 μl
75 μl
0.2 μl
125 μl
0.2 μl
0.75 ml
2 μl
1.25 ml
10 μl
7.5 ml
20 μl
If the knob is accidentally turned too far, ie is set lower
than the required value, the process must be repeated.
The approach to the set volume must always be made in
the order of decreasing value.
Operation
Place a tip on the shaft of the pipettor. Press the tip on
firmly using a slight twisting motion to ensure a positive,
airtight seal.
Note: Never handle a liquid with pipettor, which has
not been fitted with a tip.
Aspiration
• Press the pushbutton to the first positive stop, Fig. 3A.
• Holding the pipettor vertically
• Immerse the tip into the sample liquid. The depth to
which the tip is immersed in the sample liquid depends
on the model.
Model
VWR2 and VWR10
VWR20 and VWR100
VWR200 and VWR1000
VWR5000
VWR10000
• Release the pushbutton slowly and smoothly to aspi-
rate the sample, Fig. 3B.
• Wait one second and then withdraw the tip from the liquid.
• Wipe any droplets away from the outside of the tip
using a medical wipe.
Avoid touching the orifice of the tip.
Dispensing
• Place the end of the tip against the inside wall of the
vessel at an angle of 10 to 40 degrees.
• Press the pushbutton smoothly to the first stop, Fig. 3C.
• Wait one second.
• Press the pushbutton to the second stop to expel any
remaining liquid, Fig. 3D.
• Keeping the pushbutton pressed to the end, remove
the pipettor by drawing the tip along the inside surface
of the vessel.
• Release the pushbutton, Fig. 3E.
Version: 1
Issued 17/03/2014
ENGLISH
Immersion depth (mm)
≤
1
2 - 3
2 - 3
3 - 6
5 - 7
13
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