3M L25PNT Instrucciones Del Producto página 8

Troubleshooting
1. Sample fails to migrate across the strip within the first 5 minutes after application of the sample in the 3M Peanut
Protein LFD.
The sample may be too viscous and needs to be centrifuged if this was not already done during the preparation of
the sample. If sample was already centrifuged, then preparing a 1:1 dilution with the 3M Extraction Buffer may be
necessary. (Note: This may reduce the sensitivity to ~2 ppm for some matrices.)
2. A red dot appears on the test line but remainder of test line does not change color.
Sample particulate may have passed around the filter in the cassette, simply re-run the sample by taking a new 3M
Peanut Protein LFD from the kit and repeat the test.
3. The pH of extracted sample should be between 5 and 10. If pH is outside this range, further dilution may be
required (i.e., prepare a 1:1 dilution with 100 μL of the extracted sample and 100 μL of 3M Extraction Buffer. This
may reduce the sensitivity to ~2 ppm for some matrices.)
If you have questions about specific applications or procedures, please contact your 3M Food Safety representative or
distributor.
Minimum Performance Characteristics
Lowest Limit of Detection
Upper Limit of Detection
The lowest limit of detection is defined as the lowest concentration of the allergen in a test sample that can be
(a)
distinguished from a true blank sample at a specified probability level
References
1. Abbott, M., Hayward, S., Ross, W., Godefroy, S.B., Ulberth, F., Van Hengel, A. J., Roberts, J., Akiyama, H., Popping,
B., Yeung, J.M., Wehling, P., Taylor, S., Poms, R.E., and Delahaut, P. (2010). Appendix M: Validation Procedures for
Quantitative Food Allergen ELISA Methods: Community Guidance and Best Practices. J. AOAC Int. 93, 442-450.
Explanation of Symbols
www.3M.com/foodsafety/symbols
(a)
1 ppm
10 mg/mL
.
1
7
EN
(English)