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o
i
Perating
nStrUctionS
Tube
Sample Type
Color
Program 1 White
Bacteria
Yellow
Fungi
Blue
Yeast
Program 2 Orange
Soft Sample
Program 3 Red
Animal Tissue
Green
Plant Material
Program 4 Brown
Soil and
Environmental
Samples
Program 5
Cryogenically
Frozen Samples
*The preset programs are not limited to the tubes listed. You should run a test to determin the optimal
settings for other size tubes.
b. Factory Preset Program buttons:
i. The Program buttons have been designed to effectively grind various
types of samples and cannot be re-programmed.
1. To enter a program, press any of the program buttons.
a. An LED indicator will light over the selected program.
7
Tube
Speed
Time
Size
2mL
1500rpm
5 minutes
2mL
1500rpm
2 minutes
2mL
1300rpm
3 minutes
2mL
1500rpm
4 minutes
Polycar-
1600rpm
1 minute
bonate
4mL &
15mL
2. To exit program, press any other button.
3. Press the speed ON/OFF button to start program.
4. When the selected program has finished, the unit will turn off.
5. When the program is over, the display will reset itself to the
beginning of the program.
NOTE: IF THE LID IS OPENED AT ANY TIME DURING OPERATION, THE
UNIT WILL STOP AND REQUIRES THE USER TO PRESS START AGAIN TO RESTART
FUNCTION.
c. To interrupt a timing cycle, press Speed ON/OFF power button to OFF. IMPORTANT:
To completely cut off power to the unit, disconnect the power cord from the unit or
unplug from the wall outlet. If your unit does not shut off when the lid is opened,
switch the unit off and contact your Ohaus representative for repairs.
d. When the unit is not being used flip the ON/OFF power switch to the OFF position.
NOTE: IF RUNNING LESS THAN A FULL RACK OF SAMPLES, IT IS IMPORTANT TO
HAVE SAMPLES BALANCED STARTING FROM THE CENTER OF THE RACK.
IMPORTANT: To prevent unnecessary wear on the HT Lysing Homogenizer, microwell
plates and vial sample should not exceed 300 grams. A typical deep well plate with grinding
media, sample, and extraction buffer will weigh less than 200 grams.
Using sample tubes in a small tube rack with the HT Lysing Homogenizer is essentially the
same as using deep well plates. The amount of head space is very important for efficient
sample homogenization. Generally the harder the substance to be ground, the less can be
added to each tube or well. The samples must be firmly locked into the homogenizer prior
to grinding, as described under 'Operating Instructions' (see above).
Grinding times and speeds must be determined empirically.
IMPORTANT: The 4 center positions must always have tubes in them.
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