OHAUS HOHTDG Manual De Instrucciones página 9

EN
o i
Perating nStrUctionS
OPERATING TIPS
Many protocols, especially with RNA isolation, call for up to 1mL of extraction buffer with as
little as 20mg of tissue. When using 96 deep well plates, this volume is impractical. In such
situations, it is suggested that the homogenizing be performed in a smaller volume initially
and then the balance of the buffer being added after the homogenization.
The plate holder can accommodate one (1) deep well plate, four (4) stacked standard
well plates or any matrix that will fit in the 4 x 5 x 2.5" (10.2 x 12.7 x 6.4 cm) holder. Do
not run unit with any cracked or broken sample tubes or lids. In all cases, the durability
of the sample container should be tested prior to processing samples. Many brands of
polypropylene microwell plates are constructed of very thin plastic which may not tolerate
full speed with some types of grinding media.. Most deep well plates are sufficiently durable
for standard homogenizing applications.
To move the HT Lysing Homogenizer, lift it from one side until the rubber suction cup foot
on that side comes off the surface. Continue lifting on this side until all four (4) feet have
separated and the homogenizer is lying completely on its side. The homogenizer is now
ready to mount in a new location. Never run the homogenizer without the rubber suction
cup feet firmly attached. If damaged, replacement feet are available from your Ohaus
representative.
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