Simultaneous Observation In Phase Contrast + Fluorescence; Im-3F / Im-3Fl4; Im-3Ld2 / Im-3Ld4 / Im-3Ld4D - Optika Italy IM Serie Manual De Instrucciones

15. Simultaneous observation in Phase Contrast + Fluorescence

• Fluorescence models allow observation in transmitted light Phase contrast in combination with
reflected light Fluorescence. Samples with rapid decay must first be observed in Fluorescence and
then in Phase Contrast. The combined observation allows you to easily identify some areas of the
sample that emit fluorescence.

15.1 IM-3F / IM-3FL4

1. Turn on the power supply for the HBO fluorescent bulb and wait 5 minutes before the arc stabilizes.
2. Move the filter selector to an empty position or, if the filter holder is full, to the position containing the UV filter.
3. Insert the desired PH objective and move the phase contrast slider to the position containing the corresponding
phase ring.
4. Focus the sample.
5. Adjust the light intensity of the transmitted light.
6. Move the fluorescence filter selector to the desired position.
7. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate
the intensity of the fluorescence with the one of the phase contrast.

15.2 IM-3LD2 / IM-3LD4 / IM-3LD4D

1. Turn on the microscope main switch.
2. Move the filter selector to an empty position or, if the filter holder is full, to the position containing the UV filter.
3. Insert the desired PH objective and move the phase contrast slider to the position containing the corresponding
phase ring.
4. Focus the sample.
5. Adjust the light intensity of the transmitted light.
6. Move the fluorescence filter selector to the desired position.
7. Adjust the light intensity of the reflected light.
8. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate
the intensity of the fluorescence with the one of the phase contrast.
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