Use Of The Microscope In Fluorescence (Reflected Light); Centering The Mercury Bulb - Optika Italy IM Serie Manual De Instrucciones

14. Use of the microscope in fluorescence (reflected light)

14.1 Centering the mercury bulb

Only IM-3F / IM-3FL4
• Wait around 5 minutes before proceeding with this
operation to allow the bulb to properly warm up.
1. Turn on the HBO mercury bulb by operating the power
supply switch ①. (Fig. 55)
2. Turn the nosepiece into an empty position (without
objectives) and remove the protective cap, or remove
an objective from the nosepiece.
3. Place a piece of white paper on the stage and insert the
filter cube "B" into the optical path. (Fig. 56)
4. Acting on the focus screw of the collector lens ② and
on the centering screws ③ try to obtain the light spot of
the bulb's arc. (Fig. 57-58)
5. Using the focus screw of the collector lens ②, put the
image of the arc projected onto the paper. The light spot
must be brighter and sharper as possible. (Fig. 58)
①
Page 37
②
③
F ig. 55 F ig. 55
F ig. 56 F ig. 56
③
F ig. 57 F ig. 57
F ig. 58 F ig. 58