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Printed For Final Review: 10/10/03
ECO Number: 27341
12.3 Divide the corrected CPM of each calibrator, control, or unknown sample by
the corrected CPM of the Calibrator 0.
CPM of Calibrator or Unknown Sample – CPM of NSB
% B/B
=
0
12.4 Using three-cycle semi-log or log-logit graph paper, plot percent B/B
PTH calibrators (vertical axis) versus the concentration (horizontal axis).
12.5 Draw a best-fit line through the points.
12.6 Interpolate the levels of PTH in the samples from the plot.
12.7 If any unknown sample reads greater than the highest calibrator, it should be
diluted appropriately with Calibrator 0 and assayed again.
12.8 If an unknown sample has been diluted, correct for appropriate dilution factor.
12.9 Calculate the maximum binding by dividing CPM of Calibrator 0 by the
average total counts obtained in the total count tubes.
Reduction Data
The DiaSorin QC lab uses a smoothed spline curve fit.
Duplicate
Tube
CPM
Total Count
18,169
18,529
NSB
977
1,027
0 Calibrator
7,624
7,942
Calibrator (pmol/L)
1 (31.2)
7,044
6,861
2 (62.5)
6,337
6,225
3 (125)
5,362
5,424
4 (250)
4,024
3,747
5 (500)
2,658
2,754
6 (1200)
1,759
1,749
Unknown Samples
1
5,475
5,539
2
4,629
4,635
3
2,092
Typical sample data and a calibrator curve are shown in TABLE II and FIGURE 1. This
information is for reference only and should not be used for calculation of any value.
10:45 AM
CPM of Calibrator 0 – CPM of NSB
TABLE II
PTH-MM II Sample Data
Average
Corrected
Percent
CPM
CPM
18,349
1,002
7,783
6,781
6,953
5,951
6,281
5,279
5,393
4,391
3,886
2,884
2,706
1,704
1,754
752
5,507
4,505
4,632
3,630
2,103
1,101
2,113
6
x 100
0
Percent
Conc.
(B/T)
(B/B
)
(pmol/L)
0
5.5
42.4
37.9
87.8
34.2
77.8
29.4
64.8
21.2
42.5
14.7
25.1
9.6
11.1
30.0
66.4
25.2
53.5
11.5
16.2
for the
113
182
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