Use Of Fluorescence; Use Of The Polarizer (Optional) - Optika Italy B-290 Serie Manual De Instrucciones

8.10 Use of fluorescence
1. Operate on the main switch placed in the rear
side of the microscope.
• Setting on "I" turns on transmitted light, setting on
"II" turns on fluorescence. Setting on "O" turns off
the microscope. (Fig. 28)
2. Move the filter selector in the "B" position (Fig.
29) to insert the fluorescence filter in the light
path. Move the selector in the middle to work with
brightfield transmitted light.
3. Unlike a mercury lamp system, B-290LD LED illu-
mination doesn't need any power-up time for heat-
ing, and can be used immediately after switching
on. Also, the LED source is pre-aligned in factory
and doesn't need any alignment operation.
4. Focus on your sample, and adjust the light in-
tensity as needed through the brightness adjust-
ment knob. In order to improve the darkness of
the background (thus improving contrast), it is
strongly suggested to put the put a dark cover on
the light exit at the base of the microscope.
FILTER EXCITATION
NAME FILTER
B 460 - 490 nm
8.11

Use of the polarizer (optional)

1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer
until the darkest position is achieved.
3. Once the dark is achieved ("extinction" or
"Crossed Nicol" position) it is possible to begin
the observation.
DICHROIC EMISSION
MIRROR
505 nm 515LP nm
Page 18
FILTER
• FITC: fluorescent antibodies
• Achridine orange DNA - RNA
• Auramine
F ig. 28 F ig. 28
F ig. 29 F ig. 29
APPLICATIONS