Introduction; Initial Checks - Techne Hybrigene Manual Del Usuario

INTRODUCTION

Read the whole of this book before commencing work with the unit.
Blotting techniques in which DNA, RNA or protein are immobilised onto nylon or
nitrocellulose filters form a cornerstone of molecular biology. Detection of the desired
sequence is usually performed using radioactive probes. Given the expense and
hazardous nature of the probes, probing should:
a) be performed under safe conditions
b) use minimal volumes (to improve signal and reduce expense)
c) allow reuse of materials
d) be simple to use
The Techne Hybrigene meets all these requirements.
Up to five 25 x 25cm filters can be probed in one of the large hybridisation tubes.
Hybridisations can be performed in a minimal volume of 5ml, an 80% reduction over
conventional systems, reducing expense and improving signal. The operator is
protected from irradiation throughout and the apparatus is extremely simple to use.
However, the chemical manufacturer's safety precautions must be used when using
the Hybrigene.
The Techne Hybrigene also incorporates the following features:
a) a temperature range of ambient +10
b) it holds 1, 2, 3 or 4 x 80mm diameter hybridisation tubes.
c) it will hold 3, 6, 9 or 12 x 44mm diameter tubes if you use a three-tube carrier.
d) it will hold 4, 8, 12 or 16 mini tubes if you use a four-tube carrier.
e) the Hybrigene comes with adjustable feet to enable accurate levelling.

Initial Checks

When you receive your new Hybrigene remove all the packing. Check the unit
for any transit damage. If there is any transit damage at all inform Techne Sales
Department or your Techne dealer immediately.
When unpacking the unit, check that the following have been removed from the
packing: a power cable; a tube of silicon grease; a decontamination certificate
and a Guarantee Card.
Check that the unit is the correct rating for your power supply by looking on the
Rating Plate which is on the rear of the unit.
Within the guarantee period, shown on the Guarantee Card, we undertake to
supply replacements free of charge for parts which may, on examination, prove to be
defective, provided that the defect is not the result of misuse, accident or negligence.
Any instrument requiring service under this guarantee should be sent to the supplier
through whom it was purchased, or, in the case of difficulty, it should be carefully
packed in its original packing and consigned, carriage paid, to us. Techne takes no
responsibility for returned goods damaged in transit.
Returned goods will not be processed without a Returns Authorisation
Number. Call + (44) (0)1785 812121 for a number. On all correspondence, please
quote the Serial Number in full and/or the Sales Order Number. Please write the
Returns Number on the outside of any packing.
H y b r i g e n e O P E R A T O R ' S M A N U A L
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