Optika Italy IM Serie Manual De Instrucciones página 25

12. Use of the microscope in fluorescence (reflected light)
12.1 Turning on fluorescence LED
Move the main switch in the position "I" to turn on the microscope,
then rotate the adjustment knob ① (Fig. 31) to adjust the
fluorescence light intensity.
12.1.1 Switching fluorescence filter cubes
Move the filter lever (placed on the left side of the microscope)
②
to insert the desired filter cube (see tables below). (Fig. 32)
12.1.2 Available fluorescence filter cubes
• IM-300LD2
F I L T E R EXCITATION
NAME FILTER
B 455-495 nm
G 510-550 nm
• IM-300LD4 / IM-300LD4D
F I L T E R EXCITATION
NAME FILTER
M-1233 325-375 nm
M-1233.1 340-390 nm
M-1232 390-420 nm
M-1230 455-495 nm
M-1230.1 455-495 nm
M-1231 510-550 nm
M-1231.1 510-550 nm
M-1238 582-603 nm
M-1234 590-650 nm
M-1235 595-645 nm
M-1236 623-678 nm
M-1237 720-760 nm
DICHROIC EMISSION
MIRROR FILTER
500 nm 510LP nm
570 nm 575LP nm
DICHROIC EMISSION
MIRROR FILTER
415 nm 435LP nm
405 nm 420-470 nm
440 nm 450LP nm
500 nm 510LP nm
500 nm 518-542 nm
570 nm 575LP nm
570 nm 585-625 nm
610 nm 615-645 nm
660 nm 665LP nm
655 nm 665-715 nm
685 nm 690-750 nm
770 nm 780LP nm
Page 25
①
②
APPLICATIONS
• GFP, Alexa Fluor 488, Calcein, SYBR Green,
FITC, Fluo-4, MitoTracker Green
• Spectrum Gold, Propidium Iodide, Ethidium Ho-
modimer I
APPLICATIONS
• DAPI, Hoechst, NucBlue, Alexa Fluor 350, BFP,
Coumarin
• Pacific Blue, Spectrum Blue
• GFP, Alexa Fluor 488, Calcein, SYBR Green,
FITC, Fluo-4, MitoTracker Green
• Spectrum Gold, Propidium Iodide, Ethidium Ho-
modimer I
• Texas Red, Alexa Fluor 594, mRaspberry, Zom-
bie Red
• Cy5, Alexa Fluor 647, DRAQ5
• Alexa Fluor 660, DRAQ5
• Indotricarbocyanine, DiR
F ig. 31 F ig. 31
F ig. 32 F ig. 32