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Methodology Guidelines
The Use Of The Cytospin For Haematology And Other Clinical
Microscopy Specimens
Introduction
and Applications
Table 1 Uses and Applications
Uses
Romanowsky-stained cytology of CSF
and other body fluids
Gram stain and other special stains of
CSF and body fluids
Slide preparation from ficoll-hypaque
cell isolates
Cell surface and cytoplasmic marker
studies using monoclonal antibodies
Urine eosinophils
Urine hemosiderin
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Manual de operaciones de la Cytospin 4
These guidelines outlining the Use of the Cytospin for
Haematology and other Clinical Microscopy Specimens have
been prepared by:
Nicky Sherwood, MT (ASCP) and Joanne Cornbleet (MD),
Clinical Haematology Laboratory,
Stanford University Medical Centre
This section is designed to provide general guidelines for the
use of the Cytospin in haematology and clinical microscopy.
Uses
The Cytospin has a wide range of applications in clinical
microscopy, some of which are shown below.
Clinical Applications
Evaluation of possible infection or
presence of malignancy
Detection of infectious agents -
characterization of malignant cells
Provides slides for morphology,
cytochemical staining (e.g.
myeloperoxidase, non-specific
esterase), and immunocytochemical or
immunofluourescent assays (e.g.TdT).
Used to characterize leukemias and
lymphomas
Classification of leukemias and
lymphomas
Evaluation of drug-induced nephritis,
allergic cystitis, and renal transplant
rejection
Confirmation of severe intravascular
hemolysis and chronic iron overload
A78310250ES 5.1ª Edición
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