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Methodology Guidelines
The cells on the slide should have a thin layer of fluid on
their surfaces. Occasional specimens may be too thick to
completely absorb in the filter paper during a normal time
and speed setting. Such specimens may require special
processing. An example is joint aspirations that contain
hyaluronic acid, giving them a thick consistency. This
can be reduced by adding a small amount of the enzyme
hyaluronidase to the sample prior to operation of the
cytocentrifuge.
Unloading the
After the Cytospin stops, the specimens should be removed
as quickly as possible. The lid of the Cytospin is opened,
Sample Chambers
and the sealed head is removed and taken to the biological
safety cabinet. The lid is opened, and the individual sample
chamber assemblies are removed from the sealed head. The
thumb pad release lever is pressed to open the slide support.
The slide should be held horizontally on occasions where
liquid remains, allowing any residual fluid to flood the slide.
If there is a considerable amount of residual fluid, wait until
some of it evaporates. However, do not allow the specimen
to dry. Just before drying begins, place the slide in fixative,
or spray with Cell-Fixx.
For specimens that do not contain excess fluid, quickly
remove the sample chamber, remove the slide, and
immediately place into fixative. (This is best achieved by
easing the slide into the fixative, so as not to disturb the
deposited cells).
Evaluation of
After staining, the specimens can be evaluated to assess the
preparative technique.
Specimen to Assess
Technique
The ideal result is a monolayer of cells with minimal overlap,
yet sufficiently concentrated that one does not have to
search for cells in the preparation. The cells should display
excellent morphology. There should be no evidence of
stretching, or tearing of the periphery of cells. Such artefacts
indicate excessive speeds or times of cytocentrifugation.
In excellent preparations, there will be flattened nuclei
with distinct chromatin patterns. Some cell types, such as
columnar epithelial cells should retain their typical columnar
morphology. Distortion of their columnar shape indicates
A78310250ES 5.1ª Edición
Manual de operaciones de la Cytospin 4
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