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Methodology Guidelines
General Laboratory
Considerations
88
Manual de operaciones de la Cytospin 4
of cells that are easily stained and evaluated. In addition, the
construction of the Cytospin ensures maximum containment
of potentially hazardous specimens, thereby reducing the risk
to laboratory personnel.
Specimens from body fluids and all body sites can be used
for Cytospin preparations. The primary requirements
are that the specimen be a cell suspension, preferably of
single cells, and that the cells are fresh and intact enough
to yield diagnostic information. With proper application
of the general principles of Cytospin operation, consistent
preparations of well-preserved cell monolayers should result.
The Cytospin is designed to provide maximum protection to
the operator by completely containing potentially hazardous
specimens. However, Cytospin cannot protect the operator
during the various steps required to process a specimen prior
to using the Cytospin. Good laboratory practice requires
the use of a biological safety cabinet for all manipulators
of cytological specimens. This includes both the loading
and unloading steps for the Cytospin. Once the specimen
is loaded into the Cytospin sealed head, and the lid is
sealed in place, the sealed head may then be taken outside
the biological safety cabinet for spinning in the Cytospin.
After the Cytospin has stopped, the sealed head should
be returned to the biological safety cabinet prior to being
opened.
Due to the potentially infectious nature of the specimens
that may be processed in the Cytospin, the laboratory
must establish procedures to ensure that the instrument is
routinely disinfected. Suggested methods for cleaning and
disinfecting of the Cytospin and accessories will be found
in the Cleaning and Maintenance section of the Operator
Guide.
As with all clinical specimens, it is extremely important
to maintain specimen identification. For the Cytospin,
this means that the slides on to which a specimen will be
deposited must be adequately labelled with the appropriate
specimen identification. The method of labelling must
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