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EN-18
5.5. Two-Vial Methods
Some colorimetric methods require using two vials. The water sample is added to two
identical vials. One vial is being used to zero the colorimeter, referred as to the prepared
blank. A reagent is added to the other vial, referred as to the prepared sample. The
absorbance value is determined from the prepared sample.
If the method requires two or more reagents, the prepared blank could be the resulting
solution after one or more reagents have been added to the sample.
The following procedure is typical for two-vial methods:
1.
Place the prepared blank into the AP50MM sample vial compartment and
press the ZERO button to zero the instrument.
2.
Place the prepared sample into the AP50MM sample vial compartment
and press the TMR1 button to start the method timer.
3.
When the timing step completed, the measured concentration will be
displayed on the top of the page. The timing step could be terminated
earlier by pressing STOP button.
4.
Optionally, the AP50MM can re-zeroed using the prepared blank after the
timing step completed or terminated. The blank reading will subtract from
the measured concentration value, and the displayed concentration value
on the top-right corner will updated. This step is optional. It is only
necessary if the prepared blank changes its color during the timing period.
5.
Optionally, the prepared sample vial can be put back and read again by
pressing the READ button if the blank is re-zeroed after the timing step is
completed or terminated. A new concentration value based on the last
absorbance value measured will calculated and displayed.
a-AP50MM
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