Introduction; Preparation Of Single-Cell Suspensions And Homogenates From Tissues; Sample Preparation For Cell And Molecular Biology Applications - Miltenyi Biotec gentleMACS Manual Del Usuario

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Introduction

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Preparation of single-cell suspensions and homogenates from tissues

The gentleMACS™ Octo Dissociator with Heaters provides the perfect solution for the fast and easy preparation
of single-cell suspensions and homogenates from virtually any tissue. Samples are processed using unique
gentleMACS Tubes with caps that have specially designed rotors and stators. C Tubes provide gentle but
efficient tissue dissociation for cell suspensions with high viability rate. M Tubes provide thorough
homogenization of tissues or cells.
Figure 1.1: The gentleMACS Octo Dissociator with Heaters equipped with gentleMACS Tubes and Heaters.
The gentleMACS Octo Dissociator with Heaters provides:
the preparation of viable single-cell suspensions by mechanical and enzymatic dissociation, including
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incubations at 37 °C,
the preparation of thorough tissue homogenates for subsequent extraction of sub-cellular material, such as
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total RNA, DNA, proteins, organelles, and pathogens,
simultaneous or independent processing of up to eight samples, even using different programs,
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easy access to pre-defined gentleMACS Programs which are optimized for particular applications,
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the option to create user-defined programs.
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Sample preparation for cell and molecular biology applications

Two dedicated types of gentleMACS™ Tubes have been specially designed for the optimal preparation of
samples for cell biology or molecular biology applications:
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