Sample Preparation Combining Enzymatic And Mechanical Dissociation - Miltenyi Biotec gentleMACS Manual Del Usuario

Purple C Tubes are typically used for the gentle preparation of single-cell suspensions from tissues for
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subsequent cell separation, cell analysis, or cell culture.
Orange M Tubes are typically used for the homogenization of tissues and cells for subsequent subcellular or
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molecular analyses.
gentleMACS Tubes allow for:
the processing of sample volumes from 300 μL to 10 mL and from 20 mg to 4,000 mg of tissue in a single tube,
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sterile sample handling.
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gentleMACS Tubes consist of a stator and rotor element, that provides the exact sheer forces necessary to gently
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pull cells from tissues or intact molecules from cells. A specially engineered tube enclosure always directs the
sample flow towards the rotor and stator element. This sophisticated design results in highly efficient tissue
dissociation and homogenization using C Tubes and M Tubes, respectively.
Figure 1.2: Caps of the gentleMACS Tubes (exemplary, M Tubes: orange, C Tubes: purple).
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Sample preparation combining enzymatic and mechanical dissociation

For combined enzymatic and mechanical dissociation processes the instrument comes equipped with
gentleMACS™ Heaters for each of the eight tube positions. The gentleMACS Heaters allow to apply 37 °C either
to all eight positions or individually to each tube. By gently pressing the clamps, the gentleMACS Heaters can be
easily attached to the tube positions, thus connecting the gentleMACS Heaters to the instrument via its electrical
contact pins.
Figure 1.3: The gentleMACS Heaters for incubation of samples at 37 °C.
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