Command Description - Miltenyi Biotec gentleMACS Manual Del Usuario

Command
temp ON
temp OFF
spin
ramp
4
loop
end loop
Table 4.5: Overview of program commands.
Creation of a user-defined program
1. Highlight a particular User folder. In this example, the folder User_1 is selected.
2. Press Add. A new_program file template will be displayed.
3. Press the tube button to select a C Tube or M Tube.
4. To select further options, e.g., spin, ramp, temp, loop, and endloop press Add. A dotted line will appear above
the end command.
66
Description
Defines the beginning of an incubation period. Any command between the temp ON and temp
OFF command will be performed at 37 °C.
Defines the end of an incubation period. This command must be entered to stop the heating
function.
Defines the speed, direction, and duration of rotation. Speed is defined as revolutions per
minute (rpm). Prefixing the rpm value with minus (–) results in a counter-clockwise rotation.
The total duration is given in minutes and seconds.
The following are the speed restrictions:
Clockwise: +20 to +4,000 rpm
Counter-clockwise: –20 to –4,000 rpm
The change of velocity must not exceed 6,000 rpm per second.
Defines the time required to reach a certain speed. This is given in revolutions per minute
(rpm).
If a program starts with a ramp command, the starting speed will be 0 rpm.
If a ramp command is added after a spin command, the starting speed will be from the rpm of
the spin command.
Defines the beginning of a loop cycle.
Commands between the loop and end loop commands are repeatedly executed for the
defined number of cycles. A maximum of 200 cycles can be defined.
Defines the end of a loop cycle. This command must be entered as the last step of a loop cycle.