Miltenyi Biotec gentleMACS Manual Del Usuario página 62

Note: Please make sure that the tube is inserted and removed in an upright position without tilting. Tilting the
tube at an angle might result in damage of the tube seal and subsequent leakage.
Note: Ensure that the tube is securely fastened to its sleeve and socket. It should 'click' into position. Do not
force the tube into the position. In case of resistance slightly turn the tube clockwise in order to find the right
position to click in.
4 3. (Optional) If running a program including an incubation step, apply a gentleMACS™ Heater to the respective
tube position. Slightly open the clamp of the gentleMACS Heater by pressing the upper handles and adjust it
over the tube. Make sure the lower side of the gentleMACS Heater is facing towards you therefore allowing the
electrical contact to close tightly. The instrument will automatically detect the attached gentleMACS Heater.
Note: Make sure that the gentleMACS Heater is securely fastened around the tube and to the socket. Do not
use the gentleMACS Heater without a tube.
4. To select a dissociation program, press the Folder icon to select the required folder.
Note: Programs can be selected from a total of eight folders: Favorites, Miltenyi, Templates, and five User
folders. Press the folder icon several times to scroll through.
5. The list of gentleMACS Programs in the respective folder will be displayed.
6. Highlight the required gentleMACS Program touching the screen or using the scroll buttons.
Note: Press the column header Programs to sort programs within a folder alphabetically. Press the column
header Date to sort programs by the date of creation.
7. Select the required program and press OK to confirm. The status of the tube position will change to Ready. The
timer indicates the duration of the program.
Note: Use the Clear button to reset the position from the Ready status into the Selected status to assign a new
program.
8. Press Start to commence the program. The status of the tube position will change to Running. The progress
bar and timer indicate the time remaining.
Note: After selecting the program it is possible to skip the confirmation step OK by immediately pressing Start.
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