Editor Menu; Creating A New Program - Miltenyi Biotec gentleMACS Manual Del Usuario

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4.5

Editor menu

The
Editor menu is used to
create new programs (for details, refer to section 4.5.1),
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edit programs (for details, refer to section 4.5.2),
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create program shortcuts into the Favorites folder (for details, refer to section 4.5.3),
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transfer programs between folders and to a USB stick and vice versa (for details, refer to section 4.5.4).
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Note: Programs from the Miltenyi folder are not editable. Miltenyi templates from the Templates folder can
be edited but must be renamed and saved in one of the
4.5.1

Creating a new program

There are several optional commands to design a user-defined program, including options on the temperature,
rotation speed, direction, and duration, acceleration, and iteration. An overview of program commands is
shown in
Table 4.5 .
Tips and hints for user-defined programs:
Do not exceed a maximum of 3,200 rounds per run, when creating a new program. Running higher numbers
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of rounds per run may result in damage of the sealing of the gentleMACS™ Tube.
Typically, gentleMACS Programs consist of several spin commands, each with a different speed and clockwise
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rotation. Integration of short counterclockwise spin commands can increase the efficiency of the dissociation
or homogenization.
Incubation periods typically run at –20 rpm. A counter-clockwise rotation has been beneficial in many cases.
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User folders.
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