Processing Samples - Miltenyi Biotec gentleMACS Manual Del Usuario

4.4.2

Processing samples

Tips and hints for the processing of samples:
The tubes are for single use only, (single procedure).
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Only use C Tubes with the gentleMACS™ Heaters.
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Sample sizes range from 300 μL to maximal 10 mL total volume and 20 to 4,000 mg of tissue.
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The maximal amount of tissue depends on the consistency of the tissue as well as on the respective
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gentleMACS Protocol.
Dependent on the stiffness of the tissue it might be recommended to cut the sample into smaller pieces
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before processing on the gentleMACS Octo Dissociator with Heaters.
The tubes are suitable for a broad range of tissues, for example, spleen, liver, or brain. However, very hard
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material, such as bone, may damage the tubes.
For further details, refer to the respective gentleMACS Tube data sheets and to the gentleMACS Protocols at
www.gentlemacs.com .
The following instructions assume that the instrument was successfully installed and initiated as outlined in
sections 2.2 and 4.3.
To process samples, transfer an appropriate amount of tissue and buffer or other appropriate fluid into a
gentleMACS C Tube or M Tube.
Note: Tightly close the tube beyond the first resistance.
Running a single sample
Note: Do not switch off the instrument while running a program to avoid severe damage of the instrument.
1. Available tube positions are indicated as Free.
2. Attach the tube upside down to the Sleeve of an available tube position. The instrument will automatically
detect the inserted tube and assign the tube status Selected.
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