12. Fine adjust the condenser centering using the condenser
height adjustment knob, on the condenser
centering screws. (Fig. 32)
13. After properly setting the condenser, remove the centering
telescope and insert again the eyepiece. Now begin the
observation.
14. 100x objective has in internal iris diaphragm that allows the
adjustment of the numerical aperture. Rotate the diaphragm
to close the iris. (Fig. 33)
③
15. The effect one will obtain switching from a fully opened iris
(similar to a brightfield observation) to a fully closed iris (dark-
field observation) is shown in Fig. 34 and 35.
and LED
①
②
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②
①
F ig. 32
F
ig. 32
③
F ig. 33
F
ig. 33
F ig. 34
F
ig. 34
F ig. 35
F
ig. 35