Multi Wavelength mode – readings with more than one
wavelength
In the Multi Wavelength mode, absorbance values can be measured at
up to four wavelengths and the results can be mathematically processed
to obtain sums, differences and relationships.
Absorbance readings: The light absorbed by the sample is
•
measured in absorbance units.
% Transmittance measures the percent of the original light that
•
passes through the sample and reaches the detector.
Turning on the concentration factor allows selection of a specific
•
multiplier for converting absorbance readings to concentration. In a
graph of concentration versus the absorbance, the concentration
factor is the slope of the line. Concentration is calculated using a
single factor for each wavelength, which is input by the user.
Set reading mode at different wavelengths
Press Multi Wavelength in the Main Menu. Press Options for
Parameter Setup.
Table 4 Multi wavelength setup options
Options
Description
For further Options
More
Recall saved measurement data, wavelength scans or time
Recall
courses, refer to
measurement
data symbol
Switches from % transmittance to concentration or
% Trans/Abs
absorbance in reading mode.
To input the wavelength setting. Use the alphanumeric
keypad to enter the reading wavelengths. A wavelength in
Wavelength
the range of 190–1100 nm can be entered.
This functions as a stopwatch. It helps to make sure that the
steps of an analysis are correctly timed (e. g. reaction times,
wait times, etc., can be exactly specified). When the
Timer icon
specified time has elapsed, a sound is emitted. The use of
the timer has no influence on the reading program.
Multiplication factor for converting absorbance values into
Concentration
concentration values.
Factor
Concentration
To select the number of decimal places.
Resolution
Absorbance
Calculation basis for evaluating samples
Formula
To store the selected parameters as a User Program, refer to
Save as User
Single Wavelength (absorbance, concentration and
Program
transmittance readings), page
Save, recall, send and delete data, page
19.
English 21
14.