Publicidad
HistoCore SPECTRA H&E Staining System S2
3801655
CAUTION: If the HistoCore SPECTRA ST is not connected to the HistoCore SPECTRA CV, the slides will be transferred to the unload drawers. In this
occurrence, ensure there is the appropriate reagent in the unload vessel, otherwise quality of staining may be compromised.
CAUTION: Any deviations from these instructions may alter expected results and are neither validated nor supported by Leica Biosystems.
Technical Notes
100% alcohol and xylene reagents used for deparaffinization and rehydration should be rotated after every 800 slides. Reagent rotation is defined
as follows:
Empty most contaminated reagent of a series and refill container.
Move remaining container(s) up one space in sequence.
Place newly filled container into the last position of the series.
The 100% alcohol and xylene reagents used for dehydration and clearing before coverslipping also should be rotated after every 800 slides as
described above.
Two of the four 95% alcohols (steps 5 and 14) should be discarded and replaced with fresh 95% alcohol solution every 800 slides.
The two 95% dehydrating alcohols (step 16) should be discarded and replaced with fresh 95% alcohol solution every 400 slides.
If using the Reagent Management System (RMS), after change and rotation, update the RMS with changes. Instruction for use of the RMS can be
found in the HistoCore SPECTRA ST Instructions for Use.
CAUTION: Failure to rotate and change reagents as required may result in inadequate deparaffinization as well as excessive carry-over and dilution
of reagents or solvent.
NOTE: Reagent vessels (including water rinse vessels) should be inspected at periodic intervals to insure the integrity and cleanliness of the
vessels. If cleaning solutions are used, rinse vessels with adequate water to remove any residual cleaning solutions prior to use. Clean the vessels
in accordance with the instructions provided in the HistoCore SPECTRA ST Instructions for Use.
NOTE: The HistoCore SPECTRA ST Reagent Management System (RMS) tracks the number of slides that have utilized a specific station.
However, for the ancillary reagents that have been rotated, the RMS will no longer capture the total number of slides that have utilized that
specific ancillary reagent. For more information please refer to the SPECTRA ST Instructions for Use.
Table 1
Protocol Name: H&E Staining System S2
Step
Reagent
Default: Include
Oven
Oven Step
1
Xylene Dewax 1
2
Xylene Dewax 2
3
100% Alcohol Dewax 1
4
100% Alcohol Dewax 2
5
95% Alcohol Dewax 1
6
Tap Water
7
SPECTRA Hemalast S2
8
SPECTRA Hematoxylin S2
9
Tap Water
10
SPECTRA Differentiator S2
11
Tap Water
12
SPECTRA Bluing Agent S2
13
Tap Water
14
95% Alcohol Neutralizing H
15
SPECTRA Eosin S2
16*
95% Alcohol Dehyd 1
17
100% Alcohol Dhyd 1 H
18
100% Alcohol Dhyd 2 H
19
Xylene Dehyd 1
20
Xylene Dehyd 2
21
* Indicates a manually duplicated step.
Abbreviation
Oven
XylDwx 1
XylDwx 2
100Dwx 1
100Dwx 2
95Dwx 1
Tap Water
L HLst S2
L Hmtx S2
Tap Water
L Diff S2
Tap Water
L Blue S2
Tap Water
95Neutr H
L Eos S2
95Dhy 1
100Dhy 1H
100Dhy 2H
XylDhy 1
XylDhy 2
Exit
NA
Abbreviation: HE2
Time (mm:ss)
Tolerance
Process Class
15:00
50%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
Refer to
Hematoxylin
0%
Dial Settings
2:00
100%
1:00
0%
2:00
100%
2:00
100%
2:00
100%
2:00
100%
Refer to Eosin
0%
Dial Settings
2:00
0%
2:00
0%
2:00
0%
2:00
100%
2:00
100%
NA
NA
Page 3 of 75
Agitation Speed: 4
Heated Vessel
NA
NA
Dewaxing
No
Dewaxing
No
Dewaxing
No
Dewaxing
No
Dewaxing
No
NA
No
Neutralizing
No
Staining
No
NA
No
Differentiating
No
NA
No
Staining
No
NA
No
Neutralizing
No
Staining
No
Dehydrating
No
Dehydrating
No
Dehydrating
No
Dehydrating
No
Dehydrating
No
NA
NA
Oven Temperature: 70°C
Days Max
Slide Max
NA
NA
5
800
5
800
5
800
5
800
5
800
NA
NA
7
1600
7
1600
NA
NA
7
1600
NA
NA
7
1600
NA
NA
5
800
7
1600
5
400
5
800
5
800
5
800
5
800
NA
NA
Publicidad
