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HistoCore SPECTRA H&E Staining System S2
3801655
Protocol Adjustments
The validated HistoCore SPECTRA H&E Staining System S2 protocol comes pre-loaded on the HistoCore SPECTRA ST and will default to a dial setting
of 4 for both the Hematoxylin and Eosin. Only the Hematoxylin and Eosin times may be adjusted when using this HistoCore SPECTRA H&E Staining
System S2. All other protocol steps and times outlined in Table 1 are fixed. To adjust the staining intensity of the Hematoxylin and/or Eosin, use the
Dial Settings in Table 2 below.
Expected Results
By following the instruction for use, a single HistoCore SPECTRA H&E Staining System S2 should provide at least 1,600 consistent high quality stained
slides.
Recommended Controls
Routine control slides containing tissue fixed and processed in a similar manner to the test specimens should be included to ensure reagents are
performing and functioning properly.
Table 2: Dial Settings
Hematoxylin Dial Settings
Setting
Timing (mm:ss)
Setting
Dial 1
1:00
Dial 1
Dial 2
2:00
Dial 2
Dial 3
3:00
Dial 3
Dial 4
4:00
Dial 4
Dial 5
5:00
Dial 5
Dial 6
6:00
Dial 6
Dial 7
7:00
Dial 7
Dial 8
8:00
Dial 8
Dial 9
9:00
Dial 9
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Eosin Dial Settings
Timing (mm:ss)
0:30
1:00
1:30
2:00
2:30
3:00
3:30
4:00
4:30
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