Abaxis VETSCAN VS2 Manual Del Usuario página 131

°
sample tubes at 2–8 C (36–46
not be run within 60 minutes.
• Total protein and albumin results from hay-eating
horses may be altered if the sample has been taken
within a few hours of the last meal
• Total bilirubin results can be adversely affected by
5
photodegradation . Whole blood samples not run
immediately should be stored in the dark for no longer
than 60 minutes. If the sample cannot be analyzed
within that period, it should be separated into plasma
or serum and stored in a capped sample tube in the
dark at low temperatures
• Glucose concentrations are affected by the length of
time since the patient has last eaten and by the type of
sample collected from the patient. To accurately inter-
pret glucose results, samples should be obtained from
a patient who has been fasting for at least 12 hours.
Glucose concentrations in plasma and serum are typi-
cally higher than glucose levels in whole blood4.
C.7 Known Interfering Substances
• Physical interferents (hemolysis, icterus, and lipemia)
can change the reported concentrations of some ana-
lytes. The sample indices are printed in the results to
inform the operator of the levels of interferents in each
sample. If the presence of one or more interferents
causes a significant change in the concentration of a
particular analyte, that result is suppressed. See
bleshooting" on page 8-1
indices and suppressed results.
• Amylase activity can be elevated by contamination of
the sample with human salivary amylase. Salivary
amylase is found in human saliva, sweat glands, and
7
lungs .
• Potassium levels are affected by the presence of high
levels of platelets (> 1,000,000/L) or white blood
cells (> 200,000/L). Potassium can be released from
3. Carlson, GP. 1996. Clinical chemistry tests. In: B P Smith,
ed., Large Animal Internal Medicine, 2nd ed. St. Louis.
Mosby-year Book, Inc. pp. 441–469.
4. Coles, EH. 1986. Veterinary Clinical Pathology, 4th ed.
Philadelphia: W.B. Saunders Company; p. 153.
5. Sherwin, JE and R Obernolte. 1989. Bilirubin. In: LA
Kaplan and AJ Pesce, eds., Clinical Chemistry: Theory,
Analysis, and Correlation, 2nd ed. St. Louis: The C.V.
Mosby Company; pp. 1009–1015.
6. Henry, RJ, DC Cannon, and JW Winkelman. 1974. Clinical
Chemistry: Principles and Techniques, 2nd ed. New York:
Harper and Row; pp. 417–421; 1058–1059.
7. Yuan, SK and FJ Liu. 1991. Laboratory diagnosis of gastro-
intestinal tract and exocrine pancreatic disorders. In: JB
Henry, Clinical Diagnosis and Management by Laboratory
Methods, 18th ed. Philadelphia: W.B. Saunders Company;
p. 527.
°
F) if the sample can-
4
.
6
.
"Trou-
for an explanation of sample
Methods Performance
these blood constituents when the blood is allowed to
clot. This effect may also be observed when abnormal
8
cells are present .
• Potassium levels in canines, felines, and equines can
be artificially increased by potassium released from
platelets during clotting3
red blood cells do not contain significant levels of
potassium, with the exception of the Akita breed9.
Potassium levels in equines are artificially increased
by hemolysis.
• Sodium results in canines, felines, and equines may be
artificially depressed due to hyperlipidemia or severe
,
hyperproteinemia3 9.
C.8 Test Parameters
The VetScan VS2 Chemistry Analyzer operates at ambi-
ent temperatures between 15–32
sis time for all VetScan Rotors is <15 minutes. The
analyzer maintains the reagent rotor at a temperature of
° °
37 C (98.6 F) over the measurement interval.
C.9 Test Procedure
Note: Complete step-by-step operating procedures are
given in Section 4.4.
1. Remove a reagent rotor from the refrigerator. (The
rotor can be used directly from the refrigerator.
There is no need to warm up the rotor.)
2. Collect the specimen.
3. Remove the reagent rotor from its pouch. Expel any
air or air bubbles from the tip of the sample transfer
device before dispensing 90–120 L of sample or
control into the rotor through the sample port. A
90 L sample forms a line between the two arrows
etched on the rotor. If there are air bubbles in the
chamber, add more sample (up to a total of 120 L).
WARNING: Do not tap the rotor on the table or work
bench to empty the sample port, as this can damage the
rotor.
4. Touch to open the drawer. Place the rotor in
Open
the round cavity in the drawer and touch
There is no need to orient the rotor in a particular
8. Willard, xx. 1989. Electrolyte and acid-base abnormalities.
In: xx Willard, H Tvedten, and GH Turnwald, eds., Small
Animal Clinical Diagnosis by Laboratory Methods. Phila-
delphia: W.B. Saunders Company; pp. 103–120.
9. DiBartola, SP, RA Green, and HS Autran de Morais. 1994.
Electrolytes and acid-base. In: xx Willard, H Tvedten, and
GH Turnwald, eds., Small Animal Clinical Diagnosis by
Laboratory Methods, 2nd ed. Philadelphia: W.B. Saunders
Company; pp. 97–106.
,9
. Normal canine and feline
° °
C (59–90 F). Analy-
Close
.
C-5