Key Features - VWR shiroGEL Vertical 20 Manual De Instrucciones

Key Features

• Vertical screw-clamp technology - using only 4 screw-pins - dramatically
reduces assembly time; also act in conjunction with color-coded clamping bars
(see below) to distribute pressure evenly along the height of the gel to prevent
plate bowing or compression (Fig.1, number 3)
• A built-in inner buffer chamber within the PAGE insert - allows set up to be
completed without inclusion of a top tank or upper buffer chamber (Fig.1, number
6)
• Color-coded clamping bars – use green clamping bars to run up to two 2-mm-
thick gels in the standard shiroGEL VERTICAL 20 configuration, or by using the
thinner, yellow clamping bars to run up to four 2-mm-thick gels in the shiroGEL
VERTICAL 20. (Fig.1, number 4; also see section on
Converting the shiroGEL VERTICAL 20 from a 2- to 4-gel
Configuration)
• Glass plates - compress gently against a flat, level gasket to prevent current
leakage from the inner buffer chamber during electrophoresis (Fig.1, number 5);
notched glass plate is placed innermost and compresses directly against the
gasket to allow buffer to fill the upper wells within the gel, thereby facilitating
electrical connectivity between the negative electrode and the top of the gel,
whereas the plain glass plate with bonded spacers is outermost to prevent buffer
leakage from the inner chamber
• Detachable inner cooling coil - connects to the laboratory water supply or a
recirculating chiller to provide uniform, smile-free electrophoresis, while allowing
runs to be performed at higher voltage (Fig.1, number 8)
• Deep gel tank - with adequate clearance beneath the glass plates to allow a
magnetic stirrer to maintain buffer recirculation and uniform pH (Fig.1, number 9)
• Cam-caster - with very forgiving ultra-soft silicone mat compensates for glass
plate misalignment to ensure leak-free casting (Fig.1, number 10 & 11)
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