VWR shiroGEL Vertical 20 Manual De Instrucciones página 23

Loading the samples:
1. Transfer the Inner gel module containing cast gels into the main tank in the
correct orientation as indicated (+) on the module aligned with (+) on the tank, (-)
on the module aligned with (–) on the tank.
2. Fill the outer tank with 1 x reservoir buffer. See page 22 for recommended
running buffer solution. Table 6 shows the volume of buffer required.
3. Load the samples into the wells using a pipette tip taking care not to damage the
wells or induce any air bubbles.
4. Fill any unused wells with 1 X sample buffer.
5. It is a good idea to note the orientation and order the samples were loaded in.
This can be done by noting which samples were loaded adjacent to each
electrode.
Buffer Volume:
Table 5.
Buffer Volume
Minimum – Inner tank is filled to above the wells. Outer
Tank is filled to just flood the bottom of the glass plates.
Cooling potential is at a minimum which may affect
resolution.
mum – Inner tank is filled to above the wells. Outer Tank is
filled to the mum fill line. Cooling is high offering good
resolution of samples.
This may be further enhanced by using the cooling coil
shiroGEL VERTICAL 20
Total Inner Buffer
Chamber:: 640ml
Outer Tank: 1L
Total Inner Buffer
Chamber:: 640ml
Outer Tank for 2 gels: 5.4L
Outer Tank for 4 gels: 4.8L
20