Gel Preparation - VWR shiroGEL Vertical 20 Manual De Instrucciones

3. Once the resistance is overcome push
the uppermost notched glass plate into
the PAGE insert. Restore the PAGE to
its normal upright position and begin
securing the glass plates for vertical
electrophoresis, as described in Steps
3-9 of the Detailed set up of the
PAGE insert on pages 13-14.

Gel Preparation:

1. It is always advisable to work using stock solutions which allow added
convenience and save time when it comes to gel pouring. Page 22 lists stock
solutions for SDS PAGE gels which should be pre-made beforehand. For native
gel formula and running conditions, please consult a laboratory manual. The
protocol below is given for use of the standard stock solutions advised. This
should be adjusted if you are using different stock solutions or gel formulas.
2. Table 1 below shows the total volume of gel solution required. In subsequent
tables, amounts of gel and solutions are given for two 1mm thick gels so
adjustments are needed for when running single or more than two gels and for
0.75, 1.5 or 2mm thick spacers.
Table 1.
shiroGEL VERTICAL 20
For different thicknesses of gel, multiply the below amounts by the spacer
thickness.
Single – one gel, one dummy
plate
Double – two gels
Using a Triple Plate 2-gel
sandwich – four gels
Total Gel volume for a 1mm thick gel.
35ml
70ml
140ml
16