Gel Running - VWR shiroGEL Vertical 20 Manual De Instrucciones

Gel Running:

1. Fit the lid and connect to a power supply.
2. Consult Table 6, below, for details on recommended power supply voltage
settings.
3. Turn the power supply off when the loading dye reaches the bottom of the gel,
sooner if your proteins are below 4Kd in size.
4. Remove the gel running module, first emptying the inner buffer into the main tank.
Buffer can be re-used but this may affect run quality if continued.
5. Unscrew the glass plates and gently pry apart the glass plates. The gel will
usually stick to one of the plates and can be removed by first soaking in buffer
and then gently lifting with a spatula.
6. The gel is now ready to be stained with Coomassie or silver stain or the proteins
in the gel can be transferred to a membrane by electroblotting for specific band
identification and further analysis.
Table 6.
Recommended Voltages and Resultant
Current for 1mm thick, 12% gels.
2-4 gels
shiroGEL VERTICAL 20
35mA (constant); up to 350V
maximum
Runtime to 5h – no cooling; 4h
with cooling
21