3M Petrifilm 6402 Manual Del Usuario página 4

Specific Instructions for Validated Methods
AOAC® Official Methods
SM
Incubate Petrifilm RCC Plates up to 24 h ± 2 h at 35ºC ± 1ºC.
AFNOR validated method in compliance with ISO 16140
(3M-01/5-03/97 A)
Scope of the validation:
Use the following details when implementing the above Instructions for Use:
Sample preparation
Use only ISO listed diluents.
Incubation
For processed pork products and seafood incubate Petrifilm RCC Plates 14 h ± 30 min at 30ºC ± 1ºC.
For all other foods incubate Petrifilm RCC Plates 14 h ± 30 min at 35ºC ± 1ºC.
Interpretation
Calculate the number of microorganisms present in the test sample according to ISO 7218
Estimates are outside of the scope of the ISO 16140 validation.
AFNOR validated method in compliance with ISO 16140 in comparison to ISO 4832
(3M-01/5-03/97 B)
Scope of the validation:
Use the following details when implementing the above Instructions for Use:
Sample preparation
Use only ISO listed diluents.
Incubation
For processed pork products and seafood incubate Petrifilm RCC Plates 24 h ± 2 h at 30ºC ± 1ºC.
For all other foods incubate Petrifilm RCC Plates 24 h ± 2 h at 35ºC ± 1ºC.
Interpretation
Calculate the number of microorganisms present in the test sample according to ISO 7218 for one plate per dilution.
Estimates are outside of the scope of the ISO 16140 validation.
AFNOR validated method in compliance with ISO 16140 in comparison to ISO 4831
(3M-01/5-03/97 C)
Scope of the validation:
Use the following details when implementing the above Instructions for Use:
Sample preparation
Use only ISO listed diluents.
Incubation
For seafood products incubate Petrifilm RCC Plates 24 h ± 2 h at 30ºC ± 1ºC.
For all other foods incubate Petrifilm RCC Plates 24 h ± 2 h at 35ºC ± 1ºC.
Interpretation
Calculate the number of microorganisms present in the test sample according to ISO 7218 for one plate per dilution.
Estimates are outside of the scope of the ISO 16140 validation.
References
1. FDA. 1998. Bacteriological Analytical Manual, 8
2. ISO 6887-1: Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for
microbiological examination, Part 1: General rules for the preparation of the initial suspension and decimal dilutions
3. ISO 8261/IDF 122: Milk and milk products - General guidance for the preparation of test samples, initial suspensions and decimal dilutions
for microbiological examination
4. ISO 4831: Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms---
Most probable number technique
5. ISO 4832: Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coliforms--- Colony count technique
6. ISO 7218: Microbiology of food and animal feeding stuffs - General rules for microbiological examination
7. ISO 16140: Microbiology of food and animal feeding stuffs - Protocol for the validation of alternative methods
2000.15:
Dry Rehydratable Film Method for the Rapid Enumeration of coliform in Foods.
All foods
2, 3
All foods
2, 3
All foods, except processed pork products.
2, 3
ed., Revision A, Appendix 3.64.
th
in comparison to ISO 4832
7
3
5
for one plate per dilution.
6
5
4