radioimmunoassays. In: Ekins R, et al., editors.
Free Thyroid Hormones—Proceedings of the
International Symposium held in Venice, Dec
1978. Princeton: Excerpta Medica, 1979: 181-
93. 4) Caplan RH, Pagliara AS, Wickus G.
Laboratory diagnosis of hyperthyroidism: a
reappraisal. Postgraduate Medicine 1979;66:75-
90. 5) Wenzel, KW. Pharmacological
interference with in vitro tests of thyroid function.
Metabolism 1981;717-32. 6) Robbins J, Rall JE.
The iodine-containing hormones. In: Gray CH,
James VHT, editors. Hormones in blood, 3rd
edition. San Francisco: Academic Press, 1979:
576-688. 7) Witherspoon LR, El Shami AS,
Shuler SE, Neely H, Sonnemaker R, Gilbert SS,
Alyea K. Chemically blocked analog assays for
free thyronines. I. The effect of chemical
blockers on T4 analog and T4 binding by
albumin and by thyroxine-binding globulin. Clin
Chem 1988;34:9-23. 8) Burtis CA, Ashwood
ER, editors. Tietz textbook of clinical chemistry.
2nd ed. Philadelphia: Saunders, 1994: 2208. 9)
Davidsohn I, Henry JB, editors. Todd–Sanford
clinical diagnosis by laboratory methods. 14th
ed. Philadelphia: Saunders, 1969: 632. 10)
Bartalena L. Studies on thyroxine-binding
globulin. J Endocrinol Invest 1993;16:353-71.
11) Bristow AF, Gaines-Das RE, et al. The
international standard for thyroxine binding
globulin. Clin Endocrinol 1993;38:361-6. 12)
Gershengorn MC, et al. Characterization of
human thyroxine-binding globulin; evidence for a
single polypeptide chain. J Biol Chem
1977;252:8713-8. 13) Glinoer D, et al.
Regulation of maternal thyroid during
pregnancy. J Clin Endocrinol Metab
1990;71:276-87. 14) Wilke TJ. Free thyroid
hormone index, thyroid hormone / thyroxin-
binding globulin ratio, triiodothyronine uptake,
and thyroxin-binding globulin compared for
diagnostic value regarding thyroid function. Clin
Chem 1983;29:74-79.
Technical Assistance
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6
Tables and Graphs
Precision (µg/mL)
Within-Run
3
4
Mean
SD
1
3.72
0.603
2
6.98
0.820
3
7.70
0.678
4
15.7
1.66
5
27.1
2.42
6
34.3
2.17
7
71.9
4.39
Specificity
1
Compound
Thyroglobulin
Transferrin
SHBG
AFP
HSA
4
ND: Not detectable.
Method Comparison
(IML 2000) = 1.03 (IML) + 0.29 µg/mL
r = 0.981
IMMULITE 2000 TBG (PIL2KTB-18, 2010-07-07)
1
2
Total
5
CV
SD
CV
16.2%
0.79
21.2%
11.7%
1.01
14.5%
8.8%
0.91
11.8%
10.6%
1.75
11.1%
8.9%
2.74
10.1%
6.3%
2.57
7.5%
6.1%
7.71
10.7%
Amount
% Cross
2
Added
reactivity
300 ng/mL
ND
4 mg/mL
ND
80 nmol/L
ND
58 μg/mL
ND
5 mg/dL
ND
3