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HistoCore
SPECTRA
H&E Staining System
S1
3801654
S2
3801655
Materials Not Included
The HistoCore SPECTRA H&E Staining System has been designed for tissue specimens placed on non-adhesive microscope slides.
The HistoCore SPECTRA H&E Staining System requires the use of reagent grade alcohols and xylene.
Devices Required
The HistoCore SPECTRA H&E Staining System is developed and optimized exclusively for use with the HistoCore SPECTRA ST Stainer.
Storage and Stability
Store reagents at room temperature (15 - 30°C). Please follow facility guidelines for storage of flammable products.
CAUTION: Do not use after the expiration date.
NOTE: Do NOT dispose of the staining system box. It is required for scanning the box label.
In Use Stability
The HistoCore SPECTRA H&E Staining System has a 7 day in use stability and a slide capacity of 1,600 slides. After 7 days on the
HistoCore SPECTRA ST Stainer or after staining 1,600 slides, whichever occurs first, the reagents of the HistoCore SPECTRA H&E
Staining System should be disposed in accordance with local governing regulations. When the reagents are not in use, the reagent
vessels should be covered to reduce evaporation to maintain performance characteristics.
Sterility
The HistoCore SPECTRA H&E Staining System is not a sterile product.
Warnings/Precautions
A routine quality control slide(s) containing tissue fixed and processed in a similar manner to the test specimens should be performed prior
to routine use to ensure reagents are performing as intended. Reagents should be disposed of per facility guidelines for hazardous material.
Infection Material Status
The HistoCore SPECTRA H&E Staining System does not include any infectious material. However, specimens, before and after fixation,
and all materials exposed to them, should be handled as if capable of transmitting infection and disposed of with proper precautions
per facility guidelines.
Special Facilities
The HistoCore H&E Staining System does not require any use of special facilities.
Specimen Handling
Specimens intended for use with the HistoCore SPECTRA H&E Staining System should be well fixed with neutral buffered formalin.
Following processing and paraffin embedding, section tissue at a standard thickness of (2 – 5µm).
Preparation for Use
Settings for operational parameters of the HistoCore SPECTRA ST stainer and protocol schedules are provided to optimize the performance
and consistency of the HistoCore SPECTRA H&E Staining System.
Initial Set-up of HistoCore SPECTRA H&E Staining System Protocol:
Initial set up must be conducted using the supervisor mode on the HistoCore SPECTRA ST Stainer. The HistoCore SPECTRA H&E
Staining System S1/S2 protocols can be located under the "Programs" button on the left side of screen on the HistoCore
SPECTRA ST Stainer. To open, press the "Programs" button on the touch screen.
Assigning a Rack Handle Color:
•
To assign a rack handle color to the HistoCore SPECTRA H&E Staining System S1/S2 Protocol, tap the program
in the program list to select it.
•
Press the "Color" button located on the right side of the screen.
•
The color display will open on the screen. Select the desired color by pressing that color on the screen. When completed,
press the "Save" button located on the bottom right of the screen. This will save the color choice and close the window.
Oven Specifications and Staining Intensity:
The HistoCore SPECTRA H&E Staining System S1/S2 Protocols are loaded with a default setting. These default settings include
the use of the oven to dry the slides. To de-activate the use of the oven prior to staining, select the protocol under the "Programs"
tab and press the "Edit" button on the right-hand side of the screen. A new window will open displaying the oven, hematoxylin,
and eosin settings. To de-activate the oven, click the box next to "Exclude Oven Step". The hematoxylin and eosin settings are
both defaulted to 4. To increase or decrease the hematoxylin staining intensity, either increase or decrease the number on the
hematoxylin dial. To increase or decrease the eosin staining intensity, either increase or decrease the number on the eosin dial.
Once changes have been made, press the "Save" button on the bottom right hand side of the window. The window will close,
and the changes will be saved.
NOTE: If the oven step is not being utilized on the instrument prior to staining, slides should be dried at 66 - 74°C for 15 - 22 minutes.
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