Main Filter; Gassing Station; Gas Mixture - CooperSurgical K-SYSTEMS L100 Serie Manual De Instrucciones

5.1.4 Main Filter

The fi lter effi ciency of the HEPA main fi lter is 99.995% of particles 0.3 μm (D.O.P. test).
The air fl ows from the main fi lter through the work chamber in a vertical unidirectional fl ow of clean air.
Immediately before reaching the tabletop, the air separates and fl ows out through the back wall, and
through the work opening. The air returns to the suction opening of the workstation passing through the
surrounding space.

5.1.5 Gassing Station

Most of the culture media that is used in assisted reproduction
techniques is sensitive to changes in pH, it is important to fl ush the
media with CO .
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The pH of the media in the test tubes and dishes may be controlled by
fl ushing with appropriate gas mixtures.
The pre-mixed CO gas supply is connected to the back of the IVF
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Workstation. It is being heated and humidifi ed by the gas washing
bottles placed in the inner wall of the IVF workstation and coming out
through the gassing nozzles built into the table plate. An incubator
hood should be placed above the nozzles at all times.
The fl ow rate can be adjusted as well as read at the display in the inner
wall
For optimal use, only distilled water should be used.

5.1.6 Gas Mixture

Follow indications from the culture media provider for correct handling of media. Culture media must be
maintained at a pH of about 7.4, this can be obtained by gassing with an atmosphere of 5% CO
The most frequently used gas mixtures are 5% CO
INSTRUCTION MANUAL FOR K-SYSTEMS L100 SERIES WORKSTATIONS (English)
-in-air, and 5% CO
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, 5% O and 90% N .
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.
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