3M Petrifilm 6402 Instrucciones Del Producto página 2

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Petrifilm
™
Rapid Coliform Count Plate
PRODUCT DESCRIPTION AND INTENDED USE
The 3M™ Petrifilm™ Rapid Coliform Count (RCC) Plate is a sample-ready-culture-medium system which contains Violet Red Bile (VRB) nutrients, a
cold-water-soluble gelling agent, a pH indicator to detect acid and a tetrazolium indicator that facilitates colony enumeration. 3M Petrifilm RCC Plates
are useful for the enumeration of coliform bacteria in the food and dairy industries. 3M Petrifilm RCC Plate components are decontaminated though
not sterilized. 3M Food Safety is certified to ISO (International Organization for Standardization) 9001 for design and manufacturing.
AOAC INTERNATIONAL and the U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) define coliforms as Gram-negative
rods, which produce acid and gas from lactose fermentation. As colonies grow on the 3M Petrifilm RCC Plate and produce acid, the pH indicator in the
plate changes from red-orange to yellow, providing a presumptive indication of coliforms. Gas trapped around coliform colonies indicates confirmed
coliforms.
ISO defines coliforms by their ability to grow in method-specific, selective media. ISO method 4832
technique, defines coliforms by colony size and acid production on VRB with lactose (VRBL) agar. On 3M Petrifilm RCC Plates these acid-producing
coliforms are indicated by yellow acid zones, or red colonies with or without gas. ISO method 4831
number (MPN) method, defines coliforms by their ability to grow and produce gas from lactose in a selective broth. On 3M Petrifilm RCC Plates these
coliforms are indicated by red colonies associated with gas. AFNOR Certification has certified 3M Petrifilm RCC Plates as a method in comparison to
ISO method 4831 and ISO method 4832
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SAFETY
The user should read, understand, and follow all safety information in the instructions for the 3M Petrifilm RCC Plate. Retain the safety instructions for
future reference.
W WARNING: Indicates a hazardous situation, which, if not avoided, could result in death or serious injury and/or property damage.
To Reduce the risks associated with exposure to biohazards and environmental contamination:
• Follow current industry standards and local regulations for disposal of biohazardous waste.
To Reduce the risks associated with release of contaminated product:
• Follow all product storage instruction contained in the instructions for use.
• Do not use beyond expiration date.
To Reduce risk associated with bacterial infection and workplace contamination:
• Perform 3M Petrifilm RCC testing in a properly equipped laboratory under the control of a skilled microbiologist.
• The user must train its personnel in current proper testing techniques: for example, Good Laboratory Practices
To Reduce the risks associated with misinterpretation of results:
• 3M has not documented 3M Petrifilm RCC Plates for use in industries other than food and beverage. For example, 3M has not documented 3M
Petrifilm RCC Plates for testing water, pharmaceuticals, or cosmetics. Acceptance of the 3M Petrifilm RCC Plate method for the testing of water
per an accepted local government regulation is at the sole discretion and responsibility of the end-user.
• 3M Petrifilm RCC Plates have not been tested with all possible food products, food processes, testing protocols or with all possible strains of
coliform or other bacteria.
• 3M Petrifilm RCC Plates do not differentiate any one coliform strain from another.
• Do not use 3M Petrifilm RCC Plates in the diagnosis of conditions in humans or animals.
Consult the Material Safety Data Sheet for additional information.
For information on documentation of product performance, visit our website at www.3M.com/foodsafety or contact your local 3M representative or
distributor.
USER RESPONSIBILITY
Users are responsible for familiarizing themselves with product instructions and information. Visit our website at www.3M.com/foodsafety, or
contact your local 3M representative or distributor for more information.
When selecting a test method, it is important to recognize that external factors such as sampling methods, testing protocols, sample preparation,
handling, and laboratory technique may influence results. The food sample itself may influence results. For example, foods with high sugar content
may increase the potential for gas production from non-coliform Enterobacteriaceae.
It is the user's responsibility in selecting any test method or product to evaluate a sufficient number of samples with the appropriate matrices and
microbial challenges to satisfy the user that the chosen test method meets the user's criteria.
It is also the user's responsibility to determine that any test methods and results meet its customers' and suppliers' requirements.
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W WARNING
, enumerating coliforms by the colony-count
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, enumerating coliforms by the most probable
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(English)
Issue Date: 2014-06
Product Instructions
6402/6412
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, ISO 7218 , or ISO 17025 .